DNA

Part:BBa_K3904406:Design

Designed by: Bernadeta Aleksandravičiūtė   Group: iGEM21_Vilnius-Lithuania   (2021-09-21)


Recombination template for ackA knockout


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 131
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 131
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Sequence has been acquired from E.coli Nissle 1917 genome (NCBI accession number: CP022686).

References